Difference between revisions of "Part:BBa K3331002"
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pgmA [Escherichia coli (strain K12)] | pgmA [Escherichia coli (strain K12)] | ||
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Encodes 2,3-bisphosphoglycerate-dependent phosphoglycerate mutase that catalyzes the interconversion of 2-phosphoglycerate and 3-phosphoglycerate. | Encodes 2,3-bisphosphoglycerate-dependent phosphoglycerate mutase that catalyzes the interconversion of 2-phosphoglycerate and 3-phosphoglycerate. | ||
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This part can catalyzes the interconversion of alpha-D-glucose 1-phosphate and D-glucose 6-phosphate. | This part can catalyzes the interconversion of alpha-D-glucose 1-phosphate and D-glucose 6-phosphate. | ||
We optimized the sequence with the codon preferred by Bacillus subtilis. In this way, it can be more efficiently expressed in Bacillus subtilis. | We optimized the sequence with the codon preferred by Bacillus subtilis. In this way, it can be more efficiently expressed in Bacillus subtilis. | ||
Latest revision as of 10:47, 27 October 2020
E.coli pgmA
pgmA [Escherichia coli (strain K12)]
Encodes 2,3-bisphosphoglycerate-dependent phosphoglycerate mutase that catalyzes the interconversion of 2-phosphoglycerate and 3-phosphoglycerate.
This part can catalyzes the interconversion of alpha-D-glucose 1-phosphate and D-glucose 6-phosphate. We optimized the sequence with the codon preferred by Bacillus subtilis. In this way, it can be more efficiently expressed in Bacillus subtilis.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Characterization
We cloned and confirmed the target fragment by PCR. The length of our fragment is 1770bp. The following results showed the successful result.
