Difference between revisions of "Part:BBa K3033014"
(We introduced a new part iBox-PAK4 to improve the part ferritin from UM_Macau team 2019, enhancing the magnetization capability of the FtnA protein.)
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<partinfo>BBa_K3033014 parameters</partinfo>
 
<partinfo>BBa_K3033014 parameters</partinfo>
 
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<partinfo>BBa K3033014 AddReview 5</partinfo>
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<I>University of Macau 2020</I>
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<h3>UM_Macau 2020: Characterization of Magnetic ability improve</h3>
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<h4>Description</h4>
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Inspired by the magnetization and control system from UM_Macau team 2019, we design our bacteria cleaning system in the aquarium and make an improvement on their part. They overexpressed ferritin in E.coli to enhance bacterial iron storage capabilities, which allow them to control E.coli movement via magnetization.
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However, the magnetization capability of Ferritin is poorly limited. We have found literature that proves that tagging this iBox-PAK4 would be enhancing the magnetization capability of the FtnA protein. Ferritin is a ubiquitous iron storage protein. Lager protein assembly that contains more mineralized iron will generate lager magnetic forces.
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Inka-PAK4, was originally described by Baskaran et. Al, which spontaneously forms needlelike crystals when expressed in mammalian cells.
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In this literature, researchers found when inkabox with PAK4cat, conformational changes allow the spontaneous crystallization of the complex, producing long rod-shaped crystals with a unit cell that has a hexagonal arrangement of subunits around a hollow channel. Engineered ferritin-containing protein crystals, which named ftn-PAK4, exert magnetic forces that are 9 orders of magnitude larger than those in previous reports.
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For bacterial expression, Robert C. Robinson has used pGEX4T1 (GE), pET28a (Novagen) and pSY5 (His tagged) as expression vectors for Inka1 and PAK4. Furthermore, the inka-PAK4 and GFP-inka-PAK4 plasmids have been transfected into HEK293T cell and have produced protein crystal successfully.
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Therefore, we add this iBox-PAK4 encoding gene to the downstream of FtnA in our construct (fig. 2) to reach a stronger magnetic force.
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<div class="col-sm-5">
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        < img class="img-fluid" src="https://2020.igem.org/File:T--UM_Macau--Ferritin_composite_sequence.png">
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        <p style="font-style: italic; font-size: 1.2rem;">Figure 2: AHL-LuxR combination mechanism</p >
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      </div>
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For more information on characterization of these parts through the burden monitor, visit our team’s wiki page: [https://2020.igem.org/Team:UM_Macau]
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Revision as of 09:43, 27 October 2020


FtnA

Derived from E.coli and synthesized through IDT. This codes for the ferratin which would be storing ferric ions for our magnetization function. A gene that originally contained in E.coli, which encodes for ferritin, a globular protein that consist of 24 subunits to form nanocage. The nanocage structure could store Fe3+ ions/Ferritin and therefore enhance the effectiveness and efficiency of E.coli magnetization for movement control.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


No part name specified with partinfo tag. University of Macau 2020

UM_Macau 2020: Characterization of Magnetic ability improve

Description

Inspired by the magnetization and control system from UM_Macau team 2019, we design our bacteria cleaning system in the aquarium and make an improvement on their part. They overexpressed ferritin in E.coli to enhance bacterial iron storage capabilities, which allow them to control E.coli movement via magnetization. However, the magnetization capability of Ferritin is poorly limited. We have found literature that proves that tagging this iBox-PAK4 would be enhancing the magnetization capability of the FtnA protein. Ferritin is a ubiquitous iron storage protein. Lager protein assembly that contains more mineralized iron will generate lager magnetic forces. Inka-PAK4, was originally described by Baskaran et. Al, which spontaneously forms needlelike crystals when expressed in mammalian cells. In this literature, researchers found when inkabox with PAK4cat, conformational changes allow the spontaneous crystallization of the complex, producing long rod-shaped crystals with a unit cell that has a hexagonal arrangement of subunits around a hollow channel. Engineered ferritin-containing protein crystals, which named ftn-PAK4, exert magnetic forces that are 9 orders of magnitude larger than those in previous reports.

For bacterial expression, Robert C. Robinson has used pGEX4T1 (GE), pET28a (Novagen) and pSY5 (His tagged) as expression vectors for Inka1 and PAK4. Furthermore, the inka-PAK4 and GFP-inka-PAK4 plasmids have been transfected into HEK293T cell and have produced protein crystal successfully. Therefore, we add this iBox-PAK4 encoding gene to the downstream of FtnA in our construct (fig. 2) to reach a stronger magnetic force. 


       < img class="img-fluid" src="https://2020.igem.org/File:T--UM_Macau--Ferritin_composite_sequence.png">

Figure 2: AHL-LuxR combination mechanism

For more information on characterization of these parts through the burden monitor, visit our team’s wiki page: [1]