Difference between revisions of "Part:BBa K3328014:Experience"

Latest revision as of 09:37, 27 October 2020

The XOR is inspired by the NIMPLY gate, it is consisted of a toehold switch and two triggers. The trigger’s core sequence is same and at the triggers’ both ends there are the nucleotide-binding domains. When input one of these triggers, the switch can turn on. And when input these two triggers at the same time, they can pair together and form a ring in the middle, as the result, the switch will still be in OFF state.

Applications of BBa_K3328014

The left figure shows that when INPUT A and INPUT B are both 0 or 1, the fluorescence of GFP is low. And the fluorescence intensity of GFP was high in the other two groups. This corresponds to the situation described in the truth table on the right. INPUT A=1 means that aTc (0.25 mg/mL) is added, INPUT B=1 means that HSL (0.1 mg/mL) is added. Error bar: SD (n=9).

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UNIQ49932ca9b61ae916-partinfo-00000000-QINU UNIQ49932ca9b61ae916-partinfo-00000001-QINU

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 https://2020.igem.org/wiki/images/thumb/7/70/T--OUC-China--part_XOR1.jpg/800px-T--OUC-China--part_XOR1.jpg
-The left figure shows that when INPUT A=1, INPUT B=0, the fluorescence of GFP is high. And the fluorescence intensity of GFP was low in the other three groups. This didn't correspond to the situation described in the truth table on the right.  INPUT A=1 means that aTc (0.25 mg/ml) is added, INPUT B=1 means that HSL (0.1 mg/ml) is added.
+The left figure shows that when INPUT A and INPUT B are both 0 or 1, the fluorescence of GFP is low. And the fluorescence intensity of GFP was high in the other two groups. This corresponds to the situation described in the truth table on the right. INPUT A=1 means that aTc (0.25 mg/mL) is added, INPUT B=1 means that HSL (0.1 mg/mL) is added. Error bar: SD (n=9).
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