Difference between revisions of "Part:BBa K3360003:Design"
 
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===Design Notes===
 
===Design Notes===
−
Encoding the IARI-SP-2 endo-beta-1,4-glucanase of Bacillus subtilis, the entire sequence has been optimized by E.coli codons, and the N-terminus is marked with GST to increase its solubility and reduce the formation of inclusion bodies.
+
Encoding the endo-beta-1,4-glucanase IARI-SP-2 of B.subtilis ,the entire sequence has been optimized by E.coli codons, and the N-terminus is marked with GST to increase its solubility and reduce the formation of inclusion bodies.
  
  
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===Source===
 
===Source===
  
−
Encoding the endo-beta-1,4-glucanase of Bacillus subtilis, the entire sequence has been optimized by E.coli codons, and the N-terminus is marked with GST to increase its solubility and reduce the formation of inclusion bodies.
+
Encoding the endo-beta-1,4-glucanase IARI-SP-2 of B.subtilis ,the entire sequence has been optimized by E.coli codons, and the N-terminus is marked with GST to increase its solubility and reduce the formation of inclusion bodies.
  
 
===References===
 
===References===

Latest revision as of 09:22, 27 October 2020


IARI-SP-2


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1587
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Encoding the endo-beta-1,4-glucanase IARI-SP-2 of B.subtilis ,the entire sequence has been optimized by E.coli codons, and the N-terminus is marked with GST to increase its solubility and reduce the formation of inclusion bodies.


Source

Encoding the endo-beta-1,4-glucanase IARI-SP-2 of B.subtilis ,the entire sequence has been optimized by E.coli codons, and the N-terminus is marked with GST to increase its solubility and reduce the formation of inclusion bodies.

References