Difference between revisions of "Part:BBa K3329049"
| Line 4: | Line 4: | ||
This part produces spider fibroin that can strengthen the abrasion resistance of the artificial leather. | This part produces spider fibroin that can strengthen the abrasion resistance of the artificial leather. | ||
| + | |||
| + | It's noteworthy that, in our Protein Crosslinking design, we intended to adopt spider fibroin as the structural protein modification in order to enhance the tenacity of our artificial leather. Spider fibroin would be expressed in both P. Pastoris and E. coli, respectively. Therefore, codon optimization must be applied in order to avoid homologous recombination and fit the chassis organism. As a result, we synthesized 4 versions of genes coding the same spider fibroin sequence based on the 2 versions of codon optimization for P. Pastoris and 2 versions for the codon optimization of E. coli. Names for those 4 versions of CDS are documented from Spider fibroin optimized V1 to CBM optimized V4, in which the Spider fibroin optimized V1 and V2 are for P. Pastoris, and Spider fibroin optimized V3-4 are for E. coli. | ||
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
Latest revision as of 09:11, 27 October 2020
Spider fibroin optimized V4
This part produces spider fibroin that can strengthen the abrasion resistance of the artificial leather.
It's noteworthy that, in our Protein Crosslinking design, we intended to adopt spider fibroin as the structural protein modification in order to enhance the tenacity of our artificial leather. Spider fibroin would be expressed in both P. Pastoris and E. coli, respectively. Therefore, codon optimization must be applied in order to avoid homologous recombination and fit the chassis organism. As a result, we synthesized 4 versions of genes coding the same spider fibroin sequence based on the 2 versions of codon optimization for P. Pastoris and 2 versions for the codon optimization of E. coli. Names for those 4 versions of CDS are documented from Spider fibroin optimized V1 to CBM optimized V4, in which the Spider fibroin optimized V1 and V2 are for P. Pastoris, and Spider fibroin optimized V3-4 are for E. coli.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NotI site found at 542
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]