Difference between revisions of "Part:BBa K3329048"

 
 
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<partinfo>BBa_K3329048 short</partinfo>
 
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This part produces spider fibroin that can strengthen the abrasion resistance of the artificial leather.  
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This part produces spider fibroin that can strengthen the abrasion resistance of the artificial leather.
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It's noteworthy that, in our Protein Crosslinking design, we intended to adopt spider fibroin as the structural protein modification in order to enhance the tenacity of our artificial leather. Spider fibroin would be expressed in both P. Pastoris and E. coli, respectively. Therefore, codon optimization must be applied in order to avoid homologous recombination and fit the chassis organism. As a result, we synthesized 4 versions of genes coding the same spider fibroin sequence based on the 2 versions of codon optimization for P. Pastoris and 2 versions for the codon optimization of E. coli. Names for those 4 versions of CDS are documented from Spider fibroin optimized V1 to CBM optimized V4, in which the Spider fibroin optimized V1 and V2 are for P. Pastoris, and Spider fibroin optimized V3-4 are for E. coli.
  
 
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Latest revision as of 09:11, 27 October 2020


Spider fibroin optimized V3

This part produces spider fibroin that can strengthen the abrasion resistance of the artificial leather.

It's noteworthy that, in our Protein Crosslinking design, we intended to adopt spider fibroin as the structural protein modification in order to enhance the tenacity of our artificial leather. Spider fibroin would be expressed in both P. Pastoris and E. coli, respectively. Therefore, codon optimization must be applied in order to avoid homologous recombination and fit the chassis organism. As a result, we synthesized 4 versions of genes coding the same spider fibroin sequence based on the 2 versions of codon optimization for P. Pastoris and 2 versions for the codon optimization of E. coli. Names for those 4 versions of CDS are documented from Spider fibroin optimized V1 to CBM optimized V4, in which the Spider fibroin optimized V1 and V2 are for P. Pastoris, and Spider fibroin optimized V3-4 are for E. coli.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 799
    Illegal NotI site found at 458
    Illegal NotI site found at 530
    Illegal NotI site found at 548
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 737
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 694
  • 1000
    COMPATIBLE WITH RFC[1000]