Difference between revisions of "Part:BBa K3329015"

Revision as of 08:50, 27 October 2020

SpyCatcher003 optimized V4

This is a sub part in the SpyTag003-SpyCatcher003 system. It should be used with SpyTag003. It's noteworthy that, in our Protein Crosslinking design, we intended to link a triplication of SpyTag003 through elastin-like polypeptide. Moreover, SpyCatcher003 was expressed in both P. Pastoris and E. coli, respectively. Therefore, codon optimization must be applied in order to avoid homologous recombination and fit the chassis organism. As a result, we synthesized 9 versions of genes coding the same SpyCatcher003 sequence based on the codon optimization of P. Pastoris and 3 versions for the codon optimization of E. coli. Names for those 6 versions of CDS is documented from SpyCatcher003 optimized V1 to SpyCatcher003 optimized V6, in which the SpyCatcher003 optimized V1-V3 are for P. Pastoris, and SpyCatcher003 optimized V4-V6 are for E. coli.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal XhoI site found at 144
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

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 <partinfo>BBa_K3329015 short</partinfo>
-This is a sub part in the Spytag-catcher003 system. It should be used with SpyTag003.
+This is a sub part in the SpyTag003-SpyCatcher003 system. It should be used with SpyTag003. It's noteworthy that, in our Protein Crosslinking design, we intended to link a triplication of SpyTag003 through elastin-like polypeptide. Moreover, SpyCatcher003 was expressed in both P. Pastoris and E. coli, respectively. Therefore, codon optimization must be applied in order to avoid homologous recombination and fit the chassis organism. As a result, we synthesized 9 versions of genes coding the same SpyCatcher003 sequence based on the codon optimization of P. Pastoris and 3 versions for the codon optimization of E. coli. Names for those 6 versions of CDS is documented from SpyCatcher003 optimized V1 to SpyCatcher003 optimized V6, in which the SpyCatcher003 optimized V1-V3 are for P. Pastoris, and SpyCatcher003 optimized V4-V6 are for E. coli.
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