Difference between revisions of "Part:BBa K3447110"

 
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<partinfo>BBa_K3447110 short</partinfo>
 
<partinfo>BBa_K3447110 short</partinfo>
  
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The agrD gene encodes the signaling propeptide, and the agrB gene encodes the peptidase used to process the signaling propeptide. We use these two parts together to produce AIP constitutively.
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This is a part that can produce AIP constitutively.
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<!-- Add more about the biology of this part here
 
 
===Usage and Biology===
 
===Usage and Biology===
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The agrD gene encodes the signaling propeptide, and the agrB gene encodes the peptidase used to process the signaling propeptide. We use these two parts together to produce AIP constitutively.<br>
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Characterization
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To verify the construction of agrBD, the digestion and agarose gel electrophoresis were performed by a standard protocol (Fig. 1).
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[[Image: Digestion and electrophoresis of agrBD.jpg|thumb|center|500px|<b>Figure 1. Digestion and electrophoresis of agrBD.</b>]]<br>
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==<b>Design</b>==
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===Design Notes===
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We added some synonymous mutations to avoid part rules.<br>
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===Source===
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We found this sequence data in GenBank.<br>
  
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===References===
 
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>

Revision as of 08:38, 27 October 2020


The expression of AIP

This is a part that can produce AIP constitutively.


Usage and Biology

The agrD gene encodes the signaling propeptide, and the agrB gene encodes the peptidase used to process the signaling propeptide. We use these two parts together to produce AIP constitutively.
Characterization To verify the construction of agrBD, the digestion and agarose gel electrophoresis were performed by a standard protocol (Fig. 1).

Figure 1. Digestion and electrophoresis of agrBD.

Design

Design Notes

We added some synonymous mutations to avoid part rules.


Source

We found this sequence data in GenBank.

References

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 339
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal PstI site found at 339
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 339
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 339
  • 1000
    COMPATIBLE WITH RFC[1000]