Difference between revisions of "Part:BBa K3526005"

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<partinfo>BBa_K3526005 short</partinfo>
 
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<partinfo>BBa_K3526005 SequenceAndFeatures</partinfo>
  
 
The promoter of patzD can sense the presence of atzR protein and express EGFP protein, and then emit green fluorescence.
 
The promoter of patzD can sense the presence of atzR protein and express EGFP protein, and then emit green fluorescence.

Latest revision as of 08:36, 27 October 2020


PatzD-EGFP-T7 ter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 181
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

The promoter of patzD can sense the presence of atzR protein and express EGFP protein, and then emit green fluorescence.


Contribution

BBa_K3526005 is a bio-sensor that is regulated by the transcription factor PatzD. This part should be used with the part BBa_K3526004, which can consecutively express a transcription factor AtzR. Under the presence of cyanuric acid (CYA), the AtzR can bind CYA and regulate the promoter PatzD. As a result, the GFP is expressed and emit green fluorescence.

Figure 1
Figure 2

Engineering Success

To test the function of part BBa_K3526005, we co-transform the part BBa_K3526005 and part BBa_K3526004 into Pseudomonas putida KT2440, choose 0μM, 50μM, and 100μM cyanuric acid (CYA) to induce the system. It is found that the reaction has a specificity GFP signal when the concentration of CYA is 100μM.

Figure 3