Difference between revisions of "Part:BBa K3329003"
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<partinfo>BBa_K3329003 short</partinfo> | <partinfo>BBa_K3329003 short</partinfo> | ||
− | This is a sub part in the | + | This is a sub part in the SpyTag003-SpyCatcher003 system. It should be used with SpyCatcher003. It's noteworthy that, in our Protein Crosslinking design, we intended to link a triplication of SpyTag003 through elastin-like polypeptide. Moreover, SpyTag003 was expressed in both P. Pastoris and E. coli, respectively. Therefore, codon optimization must be applied in order to avoid homologous recombination and fit the chassis organism. As a result, we synthesized 9 versions of genes coding the same SpyTag003 sequence based on the codon optimization of P. Pastoris and 3 versions for the codon optimization of E. coli. Names for those 9 versions of CDS is documented from SpyTag003 optimized V1 to SpyTag003 optimized V9, in which the SpyTag003 optimized V1-V6 are for P. Pastoris, and SpyTag003 optimized V7-V9 are for E. coli. |
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Revision as of 08:35, 27 October 2020
SpyTag003 optimized V4
This is a sub part in the SpyTag003-SpyCatcher003 system. It should be used with SpyCatcher003. It's noteworthy that, in our Protein Crosslinking design, we intended to link a triplication of SpyTag003 through elastin-like polypeptide. Moreover, SpyTag003 was expressed in both P. Pastoris and E. coli, respectively. Therefore, codon optimization must be applied in order to avoid homologous recombination and fit the chassis organism. As a result, we synthesized 9 versions of genes coding the same SpyTag003 sequence based on the codon optimization of P. Pastoris and 3 versions for the codon optimization of E. coli. Names for those 9 versions of CDS is documented from SpyTag003 optimized V1 to SpyTag003 optimized V9, in which the SpyTag003 optimized V1-V6 are for P. Pastoris, and SpyTag003 optimized V7-V9 are for E. coli.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]