Difference between revisions of "Part:BBa K3376010"
 
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Pyruvate oxidase (SpxB) of S. sanguinis catalyzes the reaction between pyruvate and oxygen to generate acetyl phosphate and H2O2. It plays a role in antagonistic relationship against S. mutans. The E. coli Nissle carrying SpxB gene is capable of producing 2.15 mM and 1.84 mM of H2O2 per OD600 when grown in 5% and 10% glucose, respectively, compared to the background level of H2O2 production (i.e., 0.33 mM)
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<img src="https://static.igem.org/mediawiki/parts/1/18/T--Mingdao--vv11.gif" style="width:50%;">
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<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 08:27, 27 October 2020


ldhp-AQP-RBS-SpxB-Tr/pSB1C3

Pyruvate oxidase (SpxB) of S. sanguinis catalyzes the reaction between pyruvate and oxygen to generate acetyl phosphate and H2O2. It plays a role in antagonistic relationship against S. mutans. The E. coli Nissle carrying SpxB gene is capable of producing 2.15 mM and 1.84 mM of H2O2 per OD600 when grown in 5% and 10% glucose, respectively, compared to the background level of H2O2 production (i.e., 0.33 mM)



Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1352
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1271
    Illegal SapI site found at 1949
    Illegal SapI.rc site found at 2405