Difference between revisions of "Part:BBa K3365014:Design"
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===Design Notes=== | ===Design Notes=== | ||
| − | + | The restriction cutting sites are added to the fragment during PCR for easy ligation to the backbone. | |
| − | + | ||
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===Source=== | ===Source=== | ||
Revision as of 08:13, 27 October 2020
pBAD/araC upstream of RFP with inhibition unit containing target
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 1913
Illegal XhoI site found at 1922 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 979
Illegal AgeI site found at 1789
Illegal AgeI site found at 1901 - 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 961
Design Notes
The restriction cutting sites are added to the fragment during PCR for easy ligation to the backbone.
Source
The ligation of BBa_K3365006, BBa_K3365053 and BBa_K3365002 by Overlap PCR.