Difference between revisions of "Part:BBa K3629022"

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mCitrine is a monomeric green fluorescent protein that is derived from <i>Aequorea victoria.</i> mCitrine's optimal excitation wavelength is 515nm and its optimal emission wavelength is 529nm (1). mCitrine could be used to tag components and proteins within the cell which can then be visualized using fluorescence spectroscopy and microscopy.
 
mCitrine is a monomeric green fluorescent protein that is derived from <i>Aequorea victoria.</i> mCitrine's optimal excitation wavelength is 515nm and its optimal emission wavelength is 529nm (1). mCitrine could be used to tag components and proteins within the cell which can then be visualized using fluorescence spectroscopy and microscopy.
 
See [https://parts.igem.org/Part:BBa_K3629026 BBa_K3629026] for a functional high expression construct using this coding sequence.
 
See [https://parts.igem.org/Part:BBa_K3629026 BBa_K3629026] for a functional high expression construct using this coding sequence.
 +
 +
===Design===
 +
Below is the codon-optimized sequence of <i>Yarrowia lipolytica</i> mCherry for use in any expression construct. However, we have also made our own expression construct [https://parts.igem.org/Part:BBa_K3629025 BBa_K3629024], designed to allow for optimal expression of mCherry in <i>Yarrowia lipolytica</i> using the TEfin promoter [https://parts.igem.org/Part:BBa_K3629001 BBa_K3629001] and the XPR2 terminator [https://parts.igem.org/Part:BBa_K3629004 BBa_K3629004].
  
 
===Sequence and Features===
 
===Sequence and Features===

Revision as of 07:45, 27 October 2020


mCitrine codon optimized for Yarrowia lipolytica

mCitrine coding sequence codon-optimized for Yarrowia lipolytica.

Usage and Biology

mCitrine is a monomeric green fluorescent protein that is derived from Aequorea victoria. mCitrine's optimal excitation wavelength is 515nm and its optimal emission wavelength is 529nm (1). mCitrine could be used to tag components and proteins within the cell which can then be visualized using fluorescence spectroscopy and microscopy. See BBa_K3629026 for a functional high expression construct using this coding sequence.

Design

Below is the codon-optimized sequence of Yarrowia lipolytica mCherry for use in any expression construct. However, we have also made our own expression construct BBa_K3629024, designed to allow for optimal expression of mCherry in Yarrowia lipolytica using the TEfin promoter BBa_K3629001 and the XPR2 terminator BBa_K3629004.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 682
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 647

References

1. [online] https://www.fpbase.org/protein/mcitrine/ (Accessed October 26, 2020)