Difference between revisions of "Part:BBa K3384137:Design"

 
 
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__NOTOC__
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<partinfo>BBa_K3384137 short</partinfo>
  
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<partinfo>BBa_K3384137 SequenceAndFeatures</partinfo>
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===Design Notes===
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Please visit to the design of the basic part <partinfo>BBa_K3384314</partinfo> for the construction process of p<em>prm1</em> Ultra. The green fluorescent protein (GFP) was obtained by PCR amplification using engineered <em>Saccharomyces cerevisiae</em> BY4741-GFP genome as template. The terminator CYC1 was obtained by PCR amplification using pCas9 plasmid as template.
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===Source===
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<em>Saccharomyces cerevisiae</em>

Latest revision as of 07:39, 27 October 2020

pprm1 Ultra-GFP-CYC1 terminator


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1157


Design Notes

Please visit to the design of the basic part BBa_K3384314 for the construction process of pprm1 Ultra. The green fluorescent protein (GFP) was obtained by PCR amplification using engineered Saccharomyces cerevisiae BY4741-GFP genome as template. The terminator CYC1 was obtained by PCR amplification using pCas9 plasmid as template.


Source

Saccharomyces cerevisiae