Difference between revisions of "Part:BBa K3384132:Design"
 
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===Design Notes===
 
===Design Notes===
−
The promoter of <em>fig1</em> was obtained by PCR amplification using <em>Saccharomyces cerevisiae</em> BY4741 genome as template. The green fluorescent protein(GFP) was obtained by PCR amplification using engineered <em>Saccharomyces cerevisiae</em> BY4741-GFP genome as template. The terminator CYC1 was obtained by PCR amplification using pCas9 plasmid as template.
+
The promoter of <em>fig1</em> was obtained by PCR amplification using <em>Saccharomyces cerevisiae</em> BY4741 genome as template. The green fluorescent protein (GFP) was obtained by PCR amplification using engineered <em>Saccharomyces cerevisiae</em> BY4741-GFP genome as template. The terminator CYC1 was obtained by PCR amplification using pCas9 plasmid as template.
  
  

Latest revision as of 07:31, 27 October 2020

pfig1-GFP-CYC1 terminator


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 172
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1644


Design Notes

The promoter of fig1 was obtained by PCR amplification using Saccharomyces cerevisiae BY4741 genome as template. The green fluorescent protein (GFP) was obtained by PCR amplification using engineered Saccharomyces cerevisiae BY4741-GFP genome as template. The terminator CYC1 was obtained by PCR amplification using pCas9 plasmid as template.


Source

Saccharomyces cerevisiae