Difference between revisions of "Part:BBa K3384132:Design"
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+ | __NOTOC__ | ||
+ | <partinfo>BBa_K3384132 short</partinfo> | ||
+ | <partinfo>BBa_K3384132 SequenceAndFeatures</partinfo> | ||
+ | |||
+ | |||
+ | ===Design Notes=== | ||
+ | The promoter of <em>fig1</em> was obtained by PCR amplification using <em>Saccharomyces cerevisiae</em> BY4741 genome as template. The green fluorescent protein(GFP) was obtained by PCR amplification using engineered <em>Saccharomyces cerevisiae</em> BY4741-GFP genome as template. The terminator CYC1 was obtained by PCR amplification using pCas9 plasmid as template. | ||
+ | |||
+ | |||
+ | ===Source=== | ||
+ | |||
+ | <em>Saccharomyces cerevisiae</em> |
Revision as of 07:26, 27 October 2020
pfig1-GFP-CYC1 terminator
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 172
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1644
Design Notes
The promoter of fig1 was obtained by PCR amplification using Saccharomyces cerevisiae BY4741 genome as template. The green fluorescent protein(GFP) was obtained by PCR amplification using engineered Saccharomyces cerevisiae BY4741-GFP genome as template. The terminator CYC1 was obtained by PCR amplification using pCas9 plasmid as template.
Source
Saccharomyces cerevisiae