Difference between revisions of "Part:BBa K3645016"

Revision as of 07:15, 27 October 2020

sgRNA scaffold

Template of sgRNA. Use Golden Gate (BsaⅠ) to replace the template sequence with a ~20bp guide sequence. Add 'tagc' on the 5'-terminal of your guide sequence and 'aaac' on the reverse 5'-terminal. Transcription product is a single strain sgRNA for dCas9.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 7
Illegal NheI site found at 30
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 135
Illegal BsaI.rc site found at 37

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K3645016 short</partinfo>
-Template of sgRNA. Use Golden Gate (Bsa&#8544;) to replace the template sequence with a ~20bp guide sequence. Transcription product is a single strain sgRNA for dCas9.
+Template of sgRNA. Use Golden Gate (Bsa&#8544;) to replace the template sequence with a ~20bp guide sequence. Add 'tagc' on the 5'-terminal of your guide sequence and 'aaac' on the reverse 5'-terminal. Transcription product is a single strain sgRNA for dCas9.
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