Difference between revisions of "Part:BBa K3406008:Design"
Line 1: Line 1:
 
__NOTOC__
 
__NOTOC__
−
<partinfo>BBa_K2323008 short</partinfo>
+
<partinfo>BBa_K3406008 short</partinfo>
  
−
<partinfo>BBa_K2323008 SequenceAndFeatures</partinfo>
+
<partinfo>BBa_K3406008 SequenceAndFeatures</partinfo>
  
  
 
===Design Notes===
 
===Design Notes===
 
We use Recombinase Polymerase Amplification to amplify a short sequence of H1N1 genome. To maximize amplification success, the primer lengths should be ~25–35 nt, and the total amplicon size should be 80–140 bp. Primers are typically designed with melting temperatures between 54 and 67 °C. In addition, because we need to do transcription experiments downstream, a T7RNA polymerase promoter was appended to the 5ʹend of the forward primers to allow T7 transcription.
 
We use Recombinase Polymerase Amplification to amplify a short sequence of H1N1 genome. To maximize amplification success, the primer lengths should be ~25–35 nt, and the total amplicon size should be 80–140 bp. Primers are typically designed with melting temperatures between 54 and 67 °C. In addition, because we need to do transcription experiments downstream, a T7RNA polymerase promoter was appended to the 5ʹend of the forward primers to allow T7 transcription.

Revision as of 07:13, 27 October 2020

RPA forward primer for H1N1


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 31
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 31
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 31
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We use Recombinase Polymerase Amplification to amplify a short sequence of H1N1 genome. To maximize amplification success, the primer lengths should be ~25–35 nt, and the total amplicon size should be 80–140 bp. Primers are typically designed with melting temperatures between 54 and 67 °C. In addition, because we need to do transcription experiments downstream, a T7RNA polymerase promoter was appended to the 5ʹend of the forward primers to allow T7 transcription.