Difference between revisions of "Part:BBa K3629017"
| Line 3: | Line 3: | ||
<partinfo>BBa_K3629017 short</partinfo> | <partinfo>BBa_K3629017 short</partinfo> | ||
| − | + | __TOC__ | |
===Usage and Biology=== | ===Usage and Biology=== | ||
Revision as of 06:36, 27 October 2020
T. reesei EGII expression construct with gibson homology sequences and FLAG tag
Usage and Biology
Design
The native signal peptide from T. reesei was removed so it would not interfere with the fused XRP2 secretion tag native to Y. lipolytica.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal prefix found in sequence at 1
Illegal suffix found in sequence at 2835
Illegal EcoRI site found at 2698
Illegal SpeI site found at 749 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 1
Illegal EcoRI site found at 2698
Illegal NheI site found at 68
Illegal NheI site found at 810
Illegal SpeI site found at 749
Illegal SpeI site found at 2836
Illegal PstI site found at 2850
Illegal NotI site found at 7
Illegal NotI site found at 2843 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 1
Illegal EcoRI site found at 2698
Illegal BamHI site found at 2786
Illegal XhoI site found at 125 - 23INCOMPATIBLE WITH RFC[23]Illegal prefix found in sequence at 1
Illegal suffix found in sequence at 2836
Illegal EcoRI site found at 2698
Illegal SpeI site found at 749 - 25INCOMPATIBLE WITH RFC[25]Illegal prefix found in sequence at 1
Illegal EcoRI site found at 2698
Illegal XbaI site found at 16
Illegal SpeI site found at 749
Illegal SpeI site found at 2836
Illegal PstI site found at 2850
Illegal NgoMIV site found at 1496
Illegal NgoMIV site found at 2030
Illegal NgoMIV site found at 2348 - 1000COMPATIBLE WITH RFC[1000]
There is an EcoRI site within the XRP2 terminator, and an SpeI site within the EXP promoter making this part RFC10 incompatible. However, we added the BioBrick prefix and suffix so that the other enzymes (NotI, XbaI, and PstI) could be used to clone this part into an iGEM plasmid or another plasmid. This part can also be cloned through RFC1000 assembly.