Difference between revisions of "Part:BBa K3406001"
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<partinfo>BBa_K3406001 short</partinfo> | <partinfo>BBa_K3406001 short</partinfo> | ||
| + | The original CcaCas13b protein gene contains rare codons. We optimized the codon to make it could be expressed in common <i>E. coli</i> protein expression strains, such as <i>E. coli</i> BL21(DE3). | ||
<partinfo>BBa_K3406001 SequenceAndFeatures</partinfo> | <partinfo>BBa_K3406001 SequenceAndFeatures</partinfo> | ||
Latest revision as of 06:26, 27 October 2020
CcaCas13b coding sequence
The original CcaCas13b protein gene contains rare codons. We optimized the codon to make it could be expressed in common E. coli protein expression strains, such as E. coli BL21(DE3).
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 954
Illegal AgeI site found at 1806 - 1000COMPATIBLE WITH RFC[1000]