Difference between revisions of "Part:BBa K3406007"

 
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__NOTOC__
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<partinfo>BBa_K3406007 short</partinfo>
  
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LbaCas13a is a member of Cas13 protein family.It has the common characteristic of Type VI RNA-targeting CRISPR–Cas system’s proteins, which means it could be actived by target RNA and could cleave bystander single strained RNAs. This feature could be used to target RNA and thus to detect RNA and to edit RNA. Besides,different Cas13 protein has different base cutting preference,and the preference of CcaCas13b is Poly A/AC
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<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K3406007 SequenceAndFeatures</partinfo>
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===Expression and purification===
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===Characterizing===

Revision as of 06:16, 27 October 2020

6xHis-MBP-LbaCas13a

LbaCas13a is a member of Cas13 protein family.It has the common characteristic of Type VI RNA-targeting CRISPR–Cas system’s proteins, which means it could be actived by target RNA and could cleave bystander single strained RNAs. This feature could be used to target RNA and thus to detect RNA and to edit RNA. Besides,different Cas13 protein has different base cutting preference,and the preference of CcaCas13b is Poly A/AC Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 427
    Illegal BglII site found at 3417
    Illegal BglII site found at 4932
    Illegal BamHI site found at 1344
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 2987
    Illegal AgeI site found at 4064
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1658
    Illegal BsaI.rc site found at 2741

Expression and purification

Characterizing