Difference between revisions of "Part:BBa K3526005"
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BBa_K3526005 is a bio-sensor that is regulated by the transcription factor PatzD. This part should be used with the part BBa_K3526004, which can consecutively express a transcription factor AtzR. Under the presence of cyanuric acid (CYA), the AtzR can bind CYA and regulate the promoter PatzD. As a result, the GFP is expressed and emit green fluorescence. | BBa_K3526005 is a bio-sensor that is regulated by the transcription factor PatzD. This part should be used with the part BBa_K3526004, which can consecutively express a transcription factor AtzR. Under the presence of cyanuric acid (CYA), the AtzR can bind CYA and regulate the promoter PatzD. As a result, the GFP is expressed and emit green fluorescence. | ||
| − | [[File:T--Shanghai city-BBa K3526005 fig 0.png|500px|thumb|center| | + | [[File:T--Shanghai city-BBa K3526005 fig 0.png|500px|thumb|center|Figure 1]] |
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| + | [[File:T--Shanghai city-BBa K3526005 fig 00.png|500px|thumb|center|Figure 2]] | ||
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===Engineering Success=== | ===Engineering Success=== | ||
To test the function of part BBa_K3526005, we co-transform the part BBa_K3526005 and part BBa_K3526004 into Pseudomonas putida KT2440, choose 0μM, 50μM, and 100μM cyanuric acid (CYA) to induce the system. It is found that the reaction has a specificity GFP signal when the concentration of CYA is 100μM. | To test the function of part BBa_K3526005, we co-transform the part BBa_K3526005 and part BBa_K3526004 into Pseudomonas putida KT2440, choose 0μM, 50μM, and 100μM cyanuric acid (CYA) to induce the system. It is found that the reaction has a specificity GFP signal when the concentration of CYA is 100μM. | ||
[[File:T--Shanghai city-BBa K3526005 fig 1.png|500px|thumb|center|figure 3]] | [[File:T--Shanghai city-BBa K3526005 fig 1.png|500px|thumb|center|figure 3]] | ||
Revision as of 05:20, 27 October 2020
PatzD-EGFP-T7 ter
The promoter of patzD can sense the presence of atzR protein and express EGFP protein, and then emit green fluorescence.
Contribution
BBa_K3526005 is a bio-sensor that is regulated by the transcription factor PatzD. This part should be used with the part BBa_K3526004, which can consecutively express a transcription factor AtzR. Under the presence of cyanuric acid (CYA), the AtzR can bind CYA and regulate the promoter PatzD. As a result, the GFP is expressed and emit green fluorescence.
Engineering Success
To test the function of part BBa_K3526005, we co-transform the part BBa_K3526005 and part BBa_K3526004 into Pseudomonas putida KT2440, choose 0μM, 50μM, and 100μM cyanuric acid (CYA) to induce the system. It is found that the reaction has a specificity GFP signal when the concentration of CYA is 100μM.
