Difference between revisions of "Part:BBa K1346004"

Revision as of 04:40, 27 October 2020

pPRM1

Promoter endogenous to s. cerevisiae that is induced by alpha factor. Characterized on flow cytometer by measuring its mean GFP fluorescence over 8 concentrations of alpha factor.

Compared to the other alpha factor responsive promoters, pPRM1 has a low basal expression level, a broad range of expression, and the third highest maximum output, less than pAGA1 and pPCL2.

Alpha factor responsive promoters were selected from the following paper: Roberts, Christopher J., et al (2000 Feb 4). Signaling and Circuitry of Multiple MAPK Pathways Revealed by a Matrix of Global Gene Expression Profiles. Science, 287(5454):873-80. http://www.ncbi.nlm.nih.gov/pubmed/10657304.

iGEM2020_NJTech_China:

pprm1 Ultra BBa_K3384314

An improved part has been constructed. Based on the characterization results, we found that although pprm1 possesses three PREs, it has a low induced expression level which indicates a good potential for transformation. And the background expression level of this promoter is low which means it can be used to express some proteins that require lower concentrations in specific stage. Based on the literature, we learned the general effect of PREs on promoter strength. For example, an increase in the copy number of PREs can enhance the induced expression level of the promoter. We doubled the 3 × PREs sequence in natural pprm1 to futher evaluate the influence of copy number of PREs on the activity of promoters. The modified pprm1 is referred to as pprm1 Ultra which contains 6 × PREs.

Sequence and Features