Difference between revisions of "Part:BBa K3526004"

 
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A transcription factor, atzR, which can sense the presence of cyanuric acid.
 
A transcription factor, atzR, which can sense the presence of cyanuric acid.
  
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===Usage and Biology===
 
  
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===Contribution===
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<span class='h3bb'>Sequence and Features</span>
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BBa_K3526004 is a composite part contains a special transcription factor AtzR. This part should be used with the part BBa_K3526005. Under the presence of cyanuric acid (CYA), the AtzR can bind CYA and regulate the promoter PatzD. As a result, the GFP is expressed and emit green fluorescence.
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<partinfo>BBa_K3526004 SequenceAndFeatures</partinfo>
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[[File: File.png|500px|thumb|center|figure 1]]
  
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[[File: File.png|500px|thumb|center|figure 2]]
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===Functional Parameters===
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===Engineering Success===
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<partinfo>BBa_K3526004 parameters</partinfo>
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To test the function of part BBa_K3526004, we co-transform the part BBa_K3526004 and part BBa_K3526005 into Pseudomonas putida KT2440. After reading related articles, we choose 0μM, 50μM, and 100μM as the detection concentrations of CYA. After preliminary analysis, we obtained Figure 3.
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[[File: File.png|500px|thumb|center|figure 3]]
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                                                              Figure 3
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The results of the experiment show that with the presence of part BBa_K3526004 and BBa_K3526005, pseudomonas putida KT2440 can detect cyanuric acid.

Revision as of 04:31, 27 October 2020


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A transcription factor, atzR, which can sense the presence of cyanuric acid.


Contribution

BBa_K3526004 is a composite part contains a special transcription factor AtzR. This part should be used with the part BBa_K3526005. Under the presence of cyanuric acid (CYA), the AtzR can bind CYA and regulate the promoter PatzD. As a result, the GFP is expressed and emit green fluorescence.

figure 1
figure 2

Engineering Success

To test the function of part BBa_K3526004, we co-transform the part BBa_K3526004 and part BBa_K3526005 into Pseudomonas putida KT2440. After reading related articles, we choose 0μM, 50μM, and 100μM as the detection concentrations of CYA. After preliminary analysis, we obtained Figure 3.

figure 3
                                                              Figure 3

The results of the experiment show that with the presence of part BBa_K3526004 and BBa_K3526005, pseudomonas putida KT2440 can detect cyanuric acid.