Difference between revisions of "Part:BBa K3593002"
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Aptamer of α-amatoxin found in another literature using Sepharose immobilized method of SELEX. Its function of binding is characterized by ELONA and dot blot, by which we repeat the result successfully. | Aptamer of α-amatoxin found in another literature using Sepharose immobilized method of SELEX. Its function of binding is characterized by ELONA and dot blot, by which we repeat the result successfully. | ||
=Background= | =Background= | ||
| + | Amatoxins are chemicals present inside the genus Amanita and caused about 90% of mushroom poisoning. Being able to detect it before eating or in the field could possibly make a great decrease in people and animals who died because of poisonous mushrooms. Also being able to detect it in hospital can greatly help doctors in mushroom areas get correct information and do effective diagnosis to save the patient.<br> | ||
| + | Aptamers are oligonucleotides that form secondary structures, giving them the ability to bind targeted molecules, including ions or small molecules, and, in our case, amanitin. | ||
=Sequence and features= | =Sequence and features= | ||
<partinfo>BBa_K3593002 SequenceAndFeatures</partinfo> | <partinfo>BBa_K3593002 SequenceAndFeatures</partinfo> | ||
=Characterisation of this part= | =Characterisation of this part= | ||
| − | |||
==Experiment data== | ==Experiment data== | ||
| + | BBa_K3593002 is an aptamer of α-amanitin named H06. It comes from a previous literature.[1] Its binding affinity is tested via a method called ELONA(enzyme-linked oligonucleotide assay) which is from the same essay，with a similar principle to ELISA. We also conduct ELONA on it by ourselves to test it's binding. | ||
| + | [[File:T--GreatBay_SCIE--Fig.1 ELONA on H06 aptamer.png|600px|thumb|center]] | ||
| + | <p class="figure-description"><b><center>Fig.1 ELONA on H06 aptamer.</center></b></p> | ||
=References= | =References= | ||
| + | 1.Han Q, Xia X, Jing L, et al. Selection and characterization of DNA aptamer specially targeting α-amanitin in wild mushrooms. SDRP J Food Sci Technol. 2018;3(6):497-508. <br> | ||
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
===Usage and Biology=== | ===Usage and Biology=== | ||
Latest revision as of 04:20, 27 October 2020
ssDNA, aptamer for α-amanitin(H06)
Aptamer of α-amatoxin found in another literature using Sepharose immobilized method of SELEX. Its function of binding is characterized by ELONA and dot blot, by which we repeat the result successfully.
Background
Amatoxins are chemicals present inside the genus Amanita and caused about 90% of mushroom poisoning. Being able to detect it before eating or in the field could possibly make a great decrease in people and animals who died because of poisonous mushrooms. Also being able to detect it in hospital can greatly help doctors in mushroom areas get correct information and do effective diagnosis to save the patient.
Aptamers are oligonucleotides that form secondary structures, giving them the ability to bind targeted molecules, including ions or small molecules, and, in our case, amanitin.
Sequence and features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 44
Characterisation of this part
Experiment data
BBa_K3593002 is an aptamer of α-amanitin named H06. It comes from a previous literature.[1] Its binding affinity is tested via a method called ELONA(enzyme-linked oligonucleotide assay) which is from the same essay，with a similar principle to ELISA. We also conduct ELONA on it by ourselves to test it's binding.
References
1.Han Q, Xia X, Jing L, et al. Selection and characterization of DNA aptamer specially targeting α-amanitin in wild mushrooms. SDRP J Food Sci Technol. 2018;3(6):497-508.