Difference between revisions of "Part:BBa K3599004"
Line 5: Line 5:
  
 
===Overview===
 
===Overview===
−
Nitrilase coming from <i>Labrenzia aggregata</i>, catalysis of the reaction: a nitrile + 2H<sub>2</sub>O = a carboxylate + NH<sub>3</sub>. The codon usage was optimized for expression in <i>Escherichia coli</i>.
+
Nitrilase coming from <i>Labrenzia aggregata</i>, catalysis of the reaction: nitrile + 2H<sub>2</sub>O = carboxylate + NH<sub>3</sub>. The codon usage was optimized for expression in <i>Escherichia coli</i>.
  
  

Revision as of 04:10, 27 October 2020


laNIT


Overview

Nitrilase coming from Labrenzia aggregata, catalysis of the reaction: nitrile + 2H2O = carboxylate + NH3. The codon usage was optimized for expression in Escherichia coli.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Primitive Species Background

A novel alphaproteobacterium containing bacteriochlorophylla, and a proposal for reclassification of Stappia aggregata as Labrenzia aggregata comb. nov., of Stappia marina as Labrenzia marina comb. nov. and of Stappia alba as Labrenzia alba comb. nov., and emended descriptions of the genera Pannonibacter, Stappia and Roseibium, and of the species Roseibium denhamense and Roseibium hamelinense.


Design & Experience

In iGEM2020 TPR_China Project, the laNIT was expressed using pTac system, which was pre-embedded into commercial expression vector pET-28b. The whole expression cassette can be found in BBa_K3599008.


Reference

Huihui, Sun, Wenyuan, Gao, Haiyang, & Fan, et al. (2015). Cloning, purification and evaluation of the enzymatic properties of a novel arylacetonitrilase from luminiphilus syltensis nor5-1b: a potential biocatalyst for the synthesis of mandelic acid and its derivatives. Biotechnology Letters. 10.1007/s10529-015-1830-4