Difference between revisions of "Part:BBa J54131"
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BetI_regulated_promoter-GFP(A-1)
 
BetI_regulated_promoter-GFP(A-1)
  
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===Usage and Biology (added by CCA_San_Diego 2020)===
 
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
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<partinfo>BBa_J54131 SequenceAndFeatures</partinfo>
  
  
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===Functional Parameters===
 
===Functional Parameters===
 
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Revision as of 03:41, 27 October 2020

BetI_regulated_promoter-GFP(A-3)

BetI_regulated_promoter-GFP(A-1)

Usage and Biology (added by CCA_San_Diego 2020)

Author: Ayush Agrawal, Noah Zhang

Summary: Provides an overview of the mechanism and its functionality.

Documentation:

Transcriptional factor betI (Betaine inhibitor) is a part of the TetR/AcR family of transcriptional regulators, acting as a repressor of glycine betaine synthesis from choline. BetI repressor protein remains bound to DNA during induction. Exposed to choline, betI forms a complex with DNA binding to a 20-bp-long sequence over betTp and betIp (Santos-Zavaleta, 2019).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 13
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 759


Functional Parameters