Difference between revisions of "Part:BBa K3645009:Design"
Teddy Huang (Talk | contribs) (→Design Notes) |
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===Design Notes=== | ===Design Notes=== | ||
| − | This part is a fragment of original plasmid design, and contains other auxiliary parts (e.g. linkers, etc.) | + | This part is a fragment of original plasmid design, and contains other auxiliary parts (e.g. linkers, etc.). UGI is included so no extra ligation of UGI is needed |
| − | + | ||
| − | + | ||
===Source=== | ===Source=== | ||
Latest revision as of 03:24, 27 October 2020
PmCDA from Target-AID
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 671
Illegal XhoI site found at 280 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
This part is a fragment of original plasmid design, and contains other auxiliary parts (e.g. linkers, etc.). UGI is included so no extra ligation of UGI is needed
Source
Banno S, Nishida K, Arazoe T, Mitsunobu H, Kondo A. Deaminase-mediated multiplex genome editing in Escherichia coli. Nat Microbiol. 2018 Apr;3(4):423-429. doi: 10.1038/s41564-017-0102-6. Epub 2018 Feb 5. PMID: 29403014.