Difference between revisions of "Part:BBa K3467009:Experience"

 
 
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This year, we attained part from team CAU_China 2019 and improved its part INP-CEN (BBa_K3279008). By using RT-PCR, we extracted AGA2 cDNA from yeast
  
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[[File:T--CAU_China--AGA2_CEN.jpg|500px|thumb|center|'''Fig. 1''' AGA2_CEN]]
This experience page is provided so that any user may enter their experience using this part.<BR>Please enter
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how you used this part and how it worked out.
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===Applications of BBa_K3467009===
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In order to enhance yeast ability on fermentation, especially in cellulose degradation so that farmer could reuse the yeast powder to get more yeast biopesticide. We employed yeast surface performance protein AGA2. The endoglucanase fused with AGA2 protein can anchor in the yeast cell wall. We’ve already the part and inserted it into our plasmid. After we transferred AGA2-CEN part into our engineering yeast, we’ll test its endoglucanase enzyme activity. In addition, we fused a 6His-tag on the C-terminal of endoglucanase so that we can use immunofluorescence staining to identify the position of CEN protein.
 
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===User Reviews===
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<I>Username</I>
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Enter the review inofrmation here.
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Latest revision as of 02:57, 27 October 2020

This year, we attained part from team CAU_China 2019 and improved its part INP-CEN (BBa_K3279008). By using RT-PCR, we extracted AGA2 cDNA from yeast

Fig. 1 AGA2_CEN

In order to enhance yeast ability on fermentation, especially in cellulose degradation so that farmer could reuse the yeast powder to get more yeast biopesticide. We employed yeast surface performance protein AGA2. The endoglucanase fused with AGA2 protein can anchor in the yeast cell wall. We’ve already the part and inserted it into our plasmid. After we transferred AGA2-CEN part into our engineering yeast, we’ll test its endoglucanase enzyme activity. In addition, we fused a 6His-tag on the C-terminal of endoglucanase so that we can use immunofluorescence staining to identify the position of CEN protein.