Difference between revisions of "Part:BBa K3467004:Experience"

 
 
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We first attained our target genes specific designed dsRNA sequence and fused it with T7 promoter. In order to measure its RNAi effect to locust, we use RNAi expression kit producing dsRNA. Then we injected dsRNA solution with 0.8~2μg dsRNA per locust and injected DEPC water into control group. Here is our result:
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This experience page is provided so that any user may enter their experience using this part.<BR>Please enter
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how you used this part and how it worked out.
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===Applications of BBa_K3467004===
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[[File:T--CAU_China--ATPase_dsRNA_analysis.jpg|500px|thumb|center|'''Fig. 1''' RNAi injection experiment survival rate]]
  
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===User Reviews===
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However, from the plot above, even though the ATPase had the same effect with the CHS group, the DEPC (control) group also has a relatively high fatal rate. However, from the searched reference, ATPase dsRNA and TSP dsRNA also can result in high fatal rate (approximately 100%).
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<I>Username</I>
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Enter the review inofrmation here.
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Latest revision as of 02:15, 27 October 2020

We first attained our target genes specific designed dsRNA sequence and fused it with T7 promoter. In order to measure its RNAi effect to locust, we use RNAi expression kit producing dsRNA. Then we injected dsRNA solution with 0.8~2μg dsRNA per locust and injected DEPC water into control group. Here is our result:

Fig. 1 RNAi injection experiment survival rate

However, from the plot above, even though the ATPase had the same effect with the CHS group, the DEPC (control) group also has a relatively high fatal rate. However, from the searched reference, ATPase dsRNA and TSP dsRNA also can result in high fatal rate (approximately 100%).