Difference between revisions of "Part:BBa K3411030"

Revision as of 01:15, 27 October 2020

PdcuS

To analyze the amount of nitrate, Lambert_GA iGEM 2020 needed to correlate with nitrate levels to a reporter protein. Thus, we needed a promoter to respond to phosphorylated NarL and report downstream GFP, a well-characterized fluorescent protein. HKUST-Rice used the PdcuS protomer, which is supported by an article in the NCBI concerning potential promoters’ sensitivity to phosphorylated NarL levels. Phosphorylated NarL then represses the PdcuS promoter, lowering GFP levels in the presence of high levels of nitrate.

Due to a change in iGEM Parts Registry Criteria, the Lambert_GA 2020 team created biobricks Part:BBa_K3411030 (for the Pdcus promoter) because the 2015 HKUST-Rice team was not required to create the basic parts for the corresponding composite part.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K3411030 short</partinfo>
-Add stuff about the promoter.....
+To analyze the amount of nitrate, Lambert_GA iGEM 2020 needed to correlate with nitrate levels to a reporter protein. Thus, we needed a promoter to respond to phosphorylated NarL and report downstream GFP, a well-characterized fluorescent protein. HKUST-Rice used the PdcuS protomer, which is supported by an article in the NCBI concerning potential promoters’ sensitivity to phosphorylated NarL levels. Phosphorylated NarL then represses the PdcuS promoter, lowering GFP levels in the presence of high levels of nitrate.
+Due to a change in iGEM Parts Registry Criteria, the Lambert_GA 2020 team created biobricks [https://parts.igem.org/Part:BBa_K3411030 Part:BBa_K3411030]  (for the Pdcus promoter) because the 2015 HKUST-Rice team was not required to create the basic parts for the corresponding composite part.
 <!-- Add more about the biology of this part here