Difference between revisions of "Part:BBa K3338021"

 
 
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The presented composite part consists of hGLuc under the control of the synthetic promoter_1 with NF-&#954; and AP1 binding sites. The part can be used to examine the basal activity and the LPS sensitivity of the promoter. As a control CMV-hGLuc (<html><a href="https://parts.igem.org/Part:BBa_K3338017">BBa_K3338017</a></html>) can be used.
 
The presented composite part consists of hGLuc under the control of the synthetic promoter_1 with NF-&#954; and AP1 binding sites. The part can be used to examine the basal activity and the LPS sensitivity of the promoter. As a control CMV-hGLuc (<html><a href="https://parts.igem.org/Part:BBa_K3338017">BBa_K3338017</a></html>) can be used.
  
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===Sequence and Features===
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===Usage and Biology===
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<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K3338021 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K3338021 SequenceAndFeatures</partinfo>
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=Characterization=
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This part was used to assess the LPS sensitivity of the “Synthetic promoter_1 with NF-κB and AP1 binding sites”. It could be shown that the promoter is strongly induced following LPS-treatment of the transfected cells. However, the LPS-sensitivity of Synthetic promoter_2 (<html><a href=" https://parts.igem.org/Part:BBa_K3338002">BBa_K3338002</a></html>) is even higher. Comparing its basal activity with other promoters revealed an approximately 25-fold higher activity than the natural CMV promoter. A detailed documentation can be found on the page of the basic part <html><a href=" https://parts.igem.org/Part:BBa_K3338007">BBa_K3338007</a></html>.
  
  

Latest revision as of 00:48, 27 October 2020


SynthP_1-hGLuc

The presented composite part consists of hGLuc under the control of the synthetic promoter_1 with NF-κ and AP1 binding sites. The part can be used to examine the basal activity and the LPS sensitivity of the promoter. As a control CMV-hGLuc (BBa_K3338017) can be used.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1
    Illegal NotI site found at 58
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Characterization

This part was used to assess the LPS sensitivity of the “Synthetic promoter_1 with NF-κB and AP1 binding sites”. It could be shown that the promoter is strongly induced following LPS-treatment of the transfected cells. However, the LPS-sensitivity of Synthetic promoter_2 (BBa_K3338002) is even higher. Comparing its basal activity with other promoters revealed an approximately 25-fold higher activity than the natural CMV promoter. A detailed documentation can be found on the page of the basic part BBa_K3338007.