Difference between revisions of "Part:BBa K3338017"

 
 
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<i>Gaussia</i> luciferase expressed under control of the constitutively active CMV-promoter/enhancer. This part can be used as a positive control for activity tests of different promoters in mammalian cells.
 
<i>Gaussia</i> luciferase expressed under control of the constitutively active CMV-promoter/enhancer. This part can be used as a positive control for activity tests of different promoters in mammalian cells.
  
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===Sequence and Features===
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===Usage and Biology===
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<span class='h3bb'>Sequence and Features</span>
 
 
<partinfo>BBa_K3338017 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K3338017 SequenceAndFeatures</partinfo>
  
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<partinfo>BBa_K3338017 parameters</partinfo>
 
<partinfo>BBa_K3338017 parameters</partinfo>
 
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=Characterization=
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This part was used to test hGLuc expression, secretion and activity. Further, it was used as a standard for all promoter activity tests. A detailed documentation can be found on the page of the basic part <html><a href=" https://parts.igem.org/Part:BBa_K3338001">BBa_K3338001</a></html>.

Latest revision as of 00:39, 27 October 2020


CMV-hGLuc

Gaussia luciferase expressed under control of the constitutively active CMV-promoter/enhancer. This part can be used as a positive control for activity tests of different promoters in mammalian cells.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Characterization

This part was used to test hGLuc expression, secretion and activity. Further, it was used as a standard for all promoter activity tests. A detailed documentation can be found on the page of the basic part BBa_K3338001.