Difference between revisions of "Part:BBa K3338013"
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=Characterization= | =Characterization= | ||
| − | This part was used to | + | This part was used to characterize the P2A self-cleaving peptide. A detailed documentation can be found on the page of the basic part <html><a href=" https://parts.igem.org/Part:BBa_K3338003">BBa_K3338003</a></html>. |
Latest revision as of 00:33, 27 October 2020
CMV-EGFP-P2A-mCherry
This part contains an EGFP-P2A-mCherry cassette under control of the constitutively active CMV-promoter. The fluorescent proteins EGFP and mCherry can be independently detected. It was used to compare the functionality of the P2A peptide and an IRES for the expression of two proteins from one single promoter.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Characterization
This part was used to characterize the P2A self-cleaving peptide. A detailed documentation can be found on the page of the basic part BBa_K3338003.