Difference between revisions of "Part:BBa K1682018:Design"
Msindhu01824 (Talk | contribs) (→Design Notes) |
Msindhu01824 (Talk | contribs) (→Design Notes) |
||
| Line 9: | Line 9: | ||
The PdcuS promoter was chosen for its NarL binding site, repressible activity, and the absence of other protein-binding domains, suggesting a robust, orthogonal response. | The PdcuS promoter was chosen for its NarL binding site, repressible activity, and the absence of other protein-binding domains, suggesting a robust, orthogonal response. | ||
The construct was assembled using Golden Gate Assembly. | The construct was assembled using Golden Gate Assembly. | ||
| − | |||
===Source=== | ===Source=== | ||
Latest revision as of 23:37, 26 October 2020
PLTet0-1:NarL, PdcuS:sfGFP, J23109:TetR
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 2037
Illegal NheI site found at 2060 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 318
Illegal BglII site found at 486
Illegal BamHI site found at 1995
Illegal XhoI site found at 31 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 1149
Design Notes
The PdcuS promoter was chosen for its NarL binding site, repressible activity, and the absence of other protein-binding domains, suggesting a robust, orthogonal response. The construct was assembled using Golden Gate Assembly.
Source
NarL, PdcuS - E. coli MG1655 genome sfGFP,TetR-PLTet0-1 scaffold - pRS334 (courtesy of Tabor Lab, Rice Univeristy)