Difference between revisions of "Part:BBa K3425070"
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<partinfo>BBa_K3425070 short</partinfo> | <partinfo>BBa_K3425070 short</partinfo> | ||
| − | This composite part serves for display of nanobodies on the outer surface of <i>E. coli</i>. It is designed as a modular system in which nanobody module (in this case [[Part:BBa_K3425043|<partinfo>BBa_K3425043</partinfo>]]) can be easily exchanged for nanobody of different specificity. All components are tailored for <html><a href="https://parts.igem.org/Help:Standards/Assembly/Type_IIS">iGEM Type IIS assembly standard</a></html>. | + | This composite part serves for display of nanobodies on the outer surface of <i>E. coli</i>. It is designed as a modular system of, in which the nanobody module (in this case [[Part:BBa_K3425043|<partinfo>BBa_K3425043</partinfo>]]) can be easily exchanged for nanobody of different specificity. All components are tailored for <html><a href="https://parts.igem.org/Help:Standards/Assembly/Type_IIS">iGEM Type IIS assembly standard</a></html>. |
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| + | <br>[[Part:BBa_K3425042|<partinfo>BBa_K3425042</partinfo>]] is a signal peptide directing the fusion protein to the periplasm. | ||
| + | <br>[[Part:BBa_K3425043|<partinfo>BBa_K3425043</partinfo>]] is the nanobody specific for <i>Mycobacterium tuberculosis</i> biomarker HSP 16.3 ([[Part:BBa_K3425041|<partinfo>BBa_K3425041</partinfo>]]) | ||
| + | <br>[[Part:BBa_K3425044|<partinfo>BBa_K3425044</partinfo>]] can be used as an outer membrane display system for <i>E. coli</i> | ||
Revision as of 22:26, 26 October 2020
Modular outer membrane display construct for nanobody specific to HSP biomarker
This composite part serves for display of nanobodies on the outer surface of E. coli. It is designed as a modular system of, in which the nanobody module (in this case BBa_K3425043) can be easily exchanged for nanobody of different specificity. All components are tailored for iGEM Type IIS assembly standard.
BBa_K3425042 is a signal peptide directing the fusion protein to the periplasm.
BBa_K3425043 is the nanobody specific for Mycobacterium tuberculosis biomarker HSP 16.3 (BBa_K3425041)
BBa_K3425044 can be used as an outer membrane display system for E. coli
| Part number | Name | Description |
|---|---|---|
| BBa_K3425042 | pelB | signal peptide |
| BBa_K3425043 | B-F10 | HSP Nanobody |
| BBa_K3425044 | ehaA | Transmembrane domain |
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 1036
Illegal AgeI site found at 625 - 1000COMPATIBLE WITH RFC[1000]