Difference between revisions of "Part:BBa K3425070:Design"
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Registry of Standard Biological Parts | Registry of Standard Biological Parts | ||
− | BBa_K3425042 | + | <br>[[Part:BBa_K3425042|<partinfo>BBa_K3425042</partinfo>]] |
− | BBa_K3425043 | + | <br>[[Part:BBa_K3425043|<partinfo>BBa_K3425043</partinfo>]] |
− | BBa_K3425044 | + | <br>[[Part:BBa_K3425044|<partinfo>BBa_K3425044</partinfo>]] |
===References=== | ===References=== |
Revision as of 21:56, 26 October 2020
Modular outer membrane display construct for nanobody specific to HSP biomarker
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 1036
Illegal AgeI site found at 625 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
This part is composed of three BioBricks tailored for assembly by iGEM Type IIS standard:
BBa_K3425042
BBa_K3425043
BBa_K3425044
It is a modular system for the exposure of nanobodies on the outer surface of E. coli. In this case, the nanobody BBa_K3425043 is specific for HSP protein, biomarker of Mycobacterium tuberculosis. However, parts are designed so that the nanobody can be easily swapped.
Source
Registry of Standard Biological Parts
BBa_K3425042
BBa_K3425043
BBa_K3425044