Difference between revisions of "Part:BBa K3425070:Design"

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It is a modular system for the exposure of nanobodies on the outer surface of <i>E. coli</i>.
 
It is a modular system for the exposure of nanobodies on the outer surface of <i>E. coli</i>.
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In this case, the nanobody [[Part:BBa_K3425043|<partinfo>BBa_K3425043</partinfo>]] is specific for HSP protein of <i>Mycobacterium tuberculosis</i>.
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In this case, the nanobody [[Part:BBa_K3425043|<partinfo>BBa_K3425043</partinfo>]] is specific for HSP protein, biomarker of <i>Mycobacterium tuberculosis</i>. However, parts are designed so that the nanobody can be easily swapped.
  
 
===Source===
 
===Source===

Revision as of 21:56, 26 October 2020


Modular outer membrane display construct for nanobody specific to HSP biomarker


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1036
    Illegal AgeI site found at 625
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part is composed of three BioBricks tailored for assembly by iGEM Type IIS standard:
BBa_K3425042
BBa_K3425043
BBa_K3425044

It is a modular system for the exposure of nanobodies on the outer surface of E. coli. In this case, the nanobody BBa_K3425043 is specific for HSP protein, biomarker of Mycobacterium tuberculosis. However, parts are designed so that the nanobody can be easily swapped.

Source

Registry of Standard Biological Parts BBa_K3425042 BBa_K3425043 BBa_K3425044

References