Difference between revisions of "Part:BBa K2924026"
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<img src="https://2020.igem.org/wiki/images/9/9c/T--Puiching_Macau--bedsheet_chart.png">
 
<img src="https://2020.igem.org/wiki/images/9/9c/T--Puiching_Macau--bedsheet_chart.png">
 
<p> Figure 1. Vertical Burning Test using bedsheet</p>
 
<p> Figure 1. Vertical Burning Test using bedsheet</p>
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<h2>Fire Retardancy Test</h2>
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<p>  The test object was a wooden block with the size of 40*38*51mm and the average Moisture content% is 12.6%. All of the engineered  flame retardants displayed a significant improvement compared with the water control. </p>
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<img src="https://2020.igem.org/wiki/images/2/2e/T--Puiching_Macau--wood.png">
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<p> Figure 2. Fire retardant test for wood</p>
 
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Revision as of 20:35, 26 October 2020


α-s1-casein

The milk protein α-s1-casein from Bos taurus

Usage and Biology

α-s1-casein (CSN1S1), also named B5B3R8_BOVIN1is a gene coding for a protein product and has the GeneID: 282208, which originates from 2.

Caseins are among the most abundant proteins in native cow's milk. They are phosphoproteins and make up approximately 80% of total dairy protein mass. The milk proteins and their proportions are important to the parameters of milk manufacturing and the quality of milk protein. With a length of 17508 bp and 19 exons the bovine α-s1-casein gene is the most abundant one of the bovine caseins (12-15 g/L)3. The α-s1-casein protein may contain up to eight serine monophosphate residues, which cluster in a hydrophilic domain between amino acids 43–80 and, through modelling studies, are thought to be connected to a hydrophobic domain (amino acids 100–199) by helical and sheet secondary structures4. In the ability of milk to carry calcium phosphate, α-s1-casein also performs an significant part5.

The DNA sequence of the gene was acquired by reverse-translating the amino acid sequence. Further, in order to reach optimal heterologous expression in Escherichia coli the DNA sequence was designed with optimized codons and synthesized commercially.

Fire Retardancy Test

The test subject(Figure 1.) was in the size of bed sheet in 300*130mm and the average Moisture content% is 13.55%-14.37%. As demonstrated on Fig. 1, all of our engineered flame retardant displayed a significant improvement compared with the water control.

<img src="T--Puiching_Macau--bedsheet_chart.png">

Figure 1. Vertical Burning Test using bedsheet

Fire Retardancy Test

The test object was a wooden block with the size of 40*38*51mm and the average Moisture content% is 12.6%. All of the engineered flame retardants displayed a significant improvement compared with the water control.

<img src="T--Puiching_Macau--wood.png">

Figure 2. Fire retardant test for wood

</html> Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 643
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


References

[1]: https://www.uniprot.org/uniprot/B5B3R8

[2]: https://www.ncbi.nlm.nih.gov/gene/?term=282208

[3]: Boland, Mike, Harjinder Singh, and Abby Thompson, eds. Milk proteins: from expression to food. Academic Press, 2014.

[4]: Sheehy, P. A., et al. "Significance, origin and function of bovine milk proteins: the biological implications of manipulation or modification." Milk Proteins. Academic Press, 2008. 81-106.

[5]: Chanat, E., J. Cell Sci., 112, 3399-3412 (1999)