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The DNA sequence of the gene was acquired by reverse-translating the amino acid sequence. Further, in order to reach optimal heterologous expression in <i>Escherichia coli</i> the DNA sequence was designed with optimized codons and synthesized commercially.
 
The DNA sequence of the gene was acquired by reverse-translating the amino acid sequence. Further, in order to reach optimal heterologous expression in <i>Escherichia coli</i> the DNA sequence was designed with optimized codons and synthesized commercially.
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Revision as of 20:29, 26 October 2020


α-s1-casein

The milk protein α-s1-casein from Bos taurus

Usage and Biology

α-s1-casein (CSN1S1), also named B5B3R8_BOVIN1is a gene coding for a protein product and has the GeneID: 282208, which originates from 2.

Caseins are among the most abundant proteins in native cow's milk. They are phosphoproteins and make up approximately 80% of total dairy protein mass. The milk proteins and their proportions are important to the parameters of milk manufacturing and the quality of milk protein. With a length of 17508 bp and 19 exons the bovine α-s1-casein gene is the most abundant one of the bovine caseins (12-15 g/L)3. The α-s1-casein protein may contain up to eight serine monophosphate residues, which cluster in a hydrophilic domain between amino acids 43–80 and, through modelling studies, are thought to be connected to a hydrophobic domain (amino acids 100–199) by helical and sheet secondary structures4. In the ability of milk to carry calcium phosphate, α-s1-casein also performs an significant part5.

The DNA sequence of the gene was acquired by reverse-translating the amino acid sequence. Further, in order to reach optimal heterologous expression in Escherichia coli the DNA sequence was designed with optimized codons and synthesized commercially. <img src="T--Puiching_Macau--bedsheet_chart.png"> </html> Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 643
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


References

[1]: https://www.uniprot.org/uniprot/B5B3R8

[2]: https://www.ncbi.nlm.nih.gov/gene/?term=282208

[3]: Boland, Mike, Harjinder Singh, and Abby Thompson, eds. Milk proteins: from expression to food. Academic Press, 2014.

[4]: Sheehy, P. A., et al. "Significance, origin and function of bovine milk proteins: the biological implications of manipulation or modification." Milk Proteins. Academic Press, 2008. 81-106.

[5]: Chanat, E., J. Cell Sci., 112, 3399-3412 (1999)