Difference between revisions of "Part:BBa K3696017"
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<b>BBa_K3696017</b> is used in F8-X testings as well as F8-X and Cas12a combination testings, the protocol is shown below: <br> | <b>BBa_K3696017</b> is used in F8-X testings as well as F8-X and Cas12a combination testings, the protocol is shown below: <br> | ||
| − | [[File:T--OSA--protocol007.png| | + | [[File:T--OSA--protocol007.png|250px|thumb|center|F8-X protocol]]<br> |
<br> | <br> | ||
The system is put into the enzyme marker and reacted for 60 minutes at 37℃. <br> | The system is put into the enzyme marker and reacted for 60 minutes at 37℃. <br> | ||
[[File:T--OSA--F8-X Cu2+ concentration testing.png|450px|thumb|center|F8-X Cu2+ concentration testing]]<br> | [[File:T--OSA--F8-X Cu2+ concentration testing.png|450px|thumb|center|F8-X Cu2+ concentration testing]]<br> | ||
| − | |||
From the detected fluorescence, we determine that: F8-X's detection limit for Cu2+ (10 nM). <br> | From the detected fluorescence, we determine that: F8-X's detection limit for Cu2+ (10 nM). <br> | ||
<br> | <br> | ||
| − | |||
F8-X has specificity for Cu2+.<br> | F8-X has specificity for Cu2+.<br> | ||
Revision as of 19:24, 26 October 2020
A type of DNA oligonucleotide that can cleave towards its substrate only after binding to Cu2+.
BBa_K3696017 is used in F8-X testings as well as F8-X and Cas12a combination testings, the protocol is shown below:
The system is put into the enzyme marker and reacted for 60 minutes at 37℃.
From the detected fluorescence, we determine that: F8-X's detection limit for Cu2+ (10 nM).
F8-X has specificity for Cu2+.
F8-X is not suitable for Cas12a binding.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]