Difference between revisions of "Part:BBa K3696016"
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<b>BBa_K3696016</b> is used in S9 and Cas12a combination testings, the protocol is shown below: | <b>BBa_K3696016</b> is used in S9 and Cas12a combination testings, the protocol is shown below: | ||
| − | [[File:T--OSA--protocol006.png|250px|thumb|center|S9 protocol]] | + | [[File:T--OSA--protocol006.png|250px|thumb|center|S9 protocol]] <br> |
The system is put into the enzyme marker and reacted for 60 minutes at 37 degree. | The system is put into the enzyme marker and reacted for 60 minutes at 37 degree. | ||
Latest revision as of 18:52, 26 October 2020
A type of DNA oligonucleotide that can cleave towards its substrate only after binding to Mn2+.
BBa_K3696016 is used in S9 and Cas12a combination testings, the protocol is shown below:
The system is put into the enzyme marker and reacted for 60 minutes at 37 degree.
From the detected fluorescence, we determine that: S9 is suitable for Cas12a binding.
S9 has specificity for Mn2+.
S9's detection limit for Mn2+ (100 μM).
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]