Difference between revisions of "Part:BBa K3696016"

Revision as of 18:52, 26 October 2020

A type of DNA oligonucleotide that can cleave towards its substrate only after binding to Mn2+.

BBa_K3696016 is used in S9 and Cas12a combination testings, the protocol is shown below:

S9 protocol

The system is put into the enzyme marker and reacted for 60 minutes at 37 degree.

S9&Cas12a combined testing

From the detected fluorescence, we determine that: S9 is suitable for Cas12a binding.

S9 has specificity for Mn2+.

S9 specificity testing

S9's detection limit for Mn2+ (100 μM).

S9 Mn2+ concentration testing

Sequence and Features

Assembly Compatibility:

@@ Line 5: / Line 5: @@
 <b>BBa_K3696016</b> is used in S9 and Cas12a combination testings, the protocol is shown below:
-[[File:T--OSA--protocol006.png|300px|thumb|center|S9 protocol]]
+[[File:T--OSA--protocol006.png|250px|thumb|center|S9 protocol]]
-The system is put into the enzyme marker and reacted for 60 minutes at 37&#8451;.
+The system is put into the enzyme marker and reacted for 60 minutes at 37 degree.
 [[File:T--OSA--S9&Cas12a combined testing.png|450px|thumb|center|S9&Cas12a combined testing]]
-From the detected fluorescence, we determine that: S9 is suitable for Cas12a binding.
+From the detected fluorescence, we determine that: S9 is suitable for Cas12a binding. <br><br>
 S9 has specificity for Mn2+.
-[[File:T--OSA--S9 specificity testing.png|450px|thumb|center|S9 specificity testing]]
+[[File:T--OSA--S9 specificity testing.png|450px|thumb|center|S9 specificity testing]]<br><br>
 S9's detection limit for Mn2+ (100 &#956;M).