Difference between revisions of "Part:BBa K3696019"

Latest revision as of 18:27, 26 October 2020

A mutant of BBa_K3696000.

BBa_K3696019--BBa_K3696113, BBa_K3696002, BBa_K3696003 are used in 8-17 DNAzyme mutation testings, the protocol is shown below:

8-17 DNAzyme mutation test protocol

The system is put into the enzyme marker and reacted for 60 minutes at 37℃.

8-17 DNAzyme mutation testing

From the detected fluorescence, we determine that 8-17 DNAzyme mut 10 has higher activity and same detection limit as the original 8-17 DNAzyme.

Sequence and Features

Assembly Compatibility:

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 <partinfo>BBa_K3696019 short</partinfo>
-BBa_K3696019--BBa_K3696113, BBa_K3696002, BBa_K3696003 are used in 8-17 DNAzyme mutation testings. The system is put into the enzyme marker and reacted for 60 minutes at 37&#8451;. From the detected fluorescence, we determine that 8-17 DNAzyme mut 10 has higher activity and same detection limit as the original 8-17 DNAzyme.
+<b>BBa_K3696019--BBa_K3696113, BBa_K3696002, BBa_K3696003</b> are used in 8-17 DNAzyme mutation testings, the protocol is shown below: <br>
+[[File:T--OSA--protocol000.png|300px|thumb|center|8-17 DNAzyme mutation test protocol]]<br>
+<br>
+The system is put into the enzyme marker and reacted for 60 minutes at 37℃.<br>
+<br>
+[[File:T--OSA--parts000.png|450px|thumb|center|8-17 DNAzyme mutation testing]]<br>
+<br>
+From the detected fluorescence, we determine that 8-17 DNAzyme mut 10 has higher activity and same detection limit as the original 8-17 DNAzyme.<br>
+<br>
 <!-- Add more about the biology of this part here