Difference between revisions of "Part:BBa K3696015:Design"
 
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===Design Notes===
 
===Design Notes===
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The actual sequence of BBa_K3696015 is ATAGAGAATTGTAACTGAAGTAGCGCCGCCGTATAGTGAGAAAACTCACTAT rA GGAAGTTACAATTCTCTAT. Furthermore, in the sequence design of BBa_K3696015, the fluorescence group was not added to the sequence due to difficulties in synthesis.  
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The actual sequence of BBa_K3696015 is ATAGAGAATTGTAACTGAAGTAGCGCCGCCGTATAGTGAGAAAACTCACTAT rA GGAAGTTACAATTCTCTAT. "r" means the base after it is a RNA base. Furthermore, in the sequence design of BBa_K3696015, the fluorescence group was not added to the sequence due to difficulties in synthesis.  
  
  

Latest revision as of 18:27, 26 October 2020


A type of DNA oligonucleotide that can cleave towards its substrate only after binding to Pb2+.


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The actual sequence of BBa_K3696015 is ATAGAGAATTGTAACTGAAGTAGCGCCGCCGTATAGTGAGAAAACTCACTAT rA GGAAGTTACAATTCTCTAT. "r" means the base after it is a RNA base. Furthermore, in the sequence design of BBa_K3696015, the fluorescence group was not added to the sequence due to difficulties in synthesis.


Source

The sequence of BBa_K3696015 is artificially designed. We adopted the sequence from the article "Nucleic acid-cleaving catalytic DNA for sensing and therapeutics" and synthesized the part accordingly.

References