Difference between revisions of "Part:BBa K3696015"
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<b>BBa_K3696015</b> is used in GR-5 and Cas12a combination testings, the protocol is shown below:
 
<b>BBa_K3696015</b> is used in GR-5 and Cas12a combination testings, the protocol is shown below:
  
−
[[File:T--OSA--protocol005.png|200px|thumb|center|GR-5 protocol]]
+
[[File:T--OSA--protocol005.png|300px|thumb|center|GR-5 protocol]]
  
 
The system is put into the enzyme marker and reacted for 60 minutes at 37&#8451;.  
 
The system is put into the enzyme marker and reacted for 60 minutes at 37&#8451;.  

Revision as of 18:01, 26 October 2020


A type of DNA oligonucleotide that can cleave towards its substrate only after binding to Pb2+.

BBa_K3696015 is used in GR-5 and Cas12a combination testings, the protocol is shown below:

GR-5 protocol

The system is put into the enzyme marker and reacted for 60 minutes at 37℃.

GR-5&Cas12a combined testing

From the detected fluorescence, we determine that: GR-5 is not suitable for Cas12a binding.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]