Difference between revisions of "Part:BBa K3504017"
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<partinfo>BBa_K3504017 short</partinfo> | <partinfo>BBa_K3504017 short</partinfo> | ||
| − | + | <p style="color:red">NOTICE: Parts in our range for this season have been created as a part of our Phase I design of our project. These parts HAVE NOT been tested or characterized in the lab due to COVID-19-related precautionary measures. We have enriched our new parts pages with data from literature and results from our modeling and simulations. If you are intending on using this part or others in our range, please keep in mind these limitations and update these parts with data from your experimentation. Feel free to reach us at: igem.afcm@gmail.com for further inquiries.</p><br/> | |
==Part Description== | ==Part Description== | ||
Sp6 is a constitutive promoter from Sp6 bacteriophage but requires SP6 RNA polymerase When used for in vitro transcription, | Sp6 is a constitutive promoter from Sp6 bacteriophage but requires SP6 RNA polymerase When used for in vitro transcription, | ||
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SP6 promoter controls the transcription of the nsPs genes of pSFV; therefore, in vitro transcription and capping of the 5′ end are required for the expression of the gene of interest in mammalian cells. | SP6 promoter controls the transcription of the nsPs genes of pSFV; therefore, in vitro transcription and capping of the 5′ end are required for the expression of the gene of interest in mammalian cells. | ||
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<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
===Usage and Biology=== | ===Usage and Biology=== | ||
Revision as of 18:01, 26 October 2020
SP6 Promoter
NOTICE: Parts in our range for this season have been created as a part of our Phase I design of our project. These parts HAVE NOT been tested or characterized in the lab due to COVID-19-related precautionary measures. We have enriched our new parts pages with data from literature and results from our modeling and simulations. If you are intending on using this part or others in our range, please keep in mind these limitations and update these parts with data from your experimentation. Feel free to reach us at: igem.afcm@gmail.com for further inquiries.
Part Description
Sp6 is a constitutive promoter from Sp6 bacteriophage but requires SP6 RNA polymerase When used for in vitro transcription, and according to its orientation the promoter transcribes either the sense OR antisense transcript.
Usage
SP6 promoter controls the transcription of the nsPs genes of pSFV; therefore, in vitro transcription and capping of the 5′ end are required for the expression of the gene of interest in mammalian cells.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]