Difference between revisions of "Part:BBa K3696015"
| Line 9: | Line 9: | ||
The system is put into the enzyme marker and reacted for 60 minutes at 37℃. | The system is put into the enzyme marker and reacted for 60 minutes at 37℃. | ||
| − | [[File:T--OSA--GR-5&Cas12a combined testing.png|450px|thumb|center|GR-5&Cas12a combined testing]] | + | [[File:T--OSA--GR-5&Cas12a combined testing.png|450px|thumb|center|GR-5&Cas12a combined testing]] |
From the detected fluorescence, we determine that: GR-5 is not suitable for Cas12a binding. | From the detected fluorescence, we determine that: GR-5 is not suitable for Cas12a binding. | ||
Revision as of 17:59, 26 October 2020
A type of DNA oligonucleotide that can cleave towards its substrate only after binding to Pb2+.
BBa_K3696015 is used in GR-5 and Cas12a combination testings, the protocol is shown below:
The system is put into the enzyme marker and reacted for 60 minutes at 37℃.
From the detected fluorescence, we determine that: GR-5 is not suitable for Cas12a binding.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]