Difference between revisions of "Part:BBa K3396002"
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===Usage and Biology=== | ===Usage and Biology=== | ||
FRB is a kind of protein which can bind with FKBP induced by rapamycin. TRIM21 is an E3 ubiquitin-protein ligase which lead to degradation. To demonstrate that the Trim-Away alike method can be performed after replacing the DocS-Coh2 with FRB-FKBP interaction, we designed TRIM21-FRB. Here is the mechanism of the recombined TRIM21-FRB: | FRB is a kind of protein which can bind with FKBP induced by rapamycin. TRIM21 is an E3 ubiquitin-protein ligase which lead to degradation. To demonstrate that the Trim-Away alike method can be performed after replacing the DocS-Coh2 with FRB-FKBP interaction, we designed TRIM21-FRB. Here is the mechanism of the recombined TRIM21-FRB: | ||
| + | |||
1. The GFPnano tagged with FRB combines with targeted protein. | 1. The GFPnano tagged with FRB combines with targeted protein. | ||
| + | |||
2. TRIM21-DocS connect Coh2-GFPnano-target through the rapamycin induced DocS-Coh2 interaction. | 2. TRIM21-DocS connect Coh2-GFPnano-target through the rapamycin induced DocS-Coh2 interaction. | ||
| − | The targeted protein is degraded by ubiquitin-proteasome system recruited by TRIM21. | + | |
| + | 3. The targeted protein is degraded by ubiquitin-proteasome system recruited by TRIM21. | ||
Latest revision as of 17:45, 26 October 2020
TRIM21-FRB
Proving that RING domain on TRIM21 can still conduce degradation on protein through recruiting ubiquitin-proteasome after replacing its PRYSPRY with DocS, we replace the DocS with FRB.
Usage and Biology
FRB is a kind of protein which can bind with FKBP induced by rapamycin. TRIM21 is an E3 ubiquitin-protein ligase which lead to degradation. To demonstrate that the Trim-Away alike method can be performed after replacing the DocS-Coh2 with FRB-FKBP interaction, we designed TRIM21-FRB. Here is the mechanism of the recombined TRIM21-FRB:
1. The GFPnano tagged with FRB combines with targeted protein.
2. TRIM21-DocS connect Coh2-GFPnano-target through the rapamycin induced DocS-Coh2 interaction.
3. The targeted protein is degraded by ubiquitin-proteasome system recruited by TRIM21.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 204
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1088
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 161
- 1000COMPATIBLE WITH RFC[1000]