Difference between revisions of "Part:BBa K3696002"
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<b>BBa_K3696001, BBa_K3696002</b> are used in 8-17 DNAzyme testings, the protocol is shown below: <br> | <b>BBa_K3696001, BBa_K3696002</b> are used in 8-17 DNAzyme testings, the protocol is shown below: <br> | ||
| − | [[File:T--OSA--protocol002.png| | + | [[File:T--OSA--protocol002.png|300px|thumb|center|8-17 DNAzyme protocol]]<br> |
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The system is put into the enzyme marker and reacted for 60 minutes at 37℃.<br> | The system is put into the enzyme marker and reacted for 60 minutes at 37℃.<br> | ||
| − | [[File:T--OSA--parts002.png| | + | [[File:T--OSA--parts002.png|450px|thumb|center|8-17 DNAzyme(two parts) Pb2+ testing]]<br> |
From the detected fluorescence, we determine that: 8-17 DNAzyme system's detection limit for Pb2+ is 10 nM.<br> | From the detected fluorescence, we determine that: 8-17 DNAzyme system's detection limit for Pb2+ is 10 nM.<br> | ||
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Latest revision as of 17:31, 26 October 2020
The cleavable substrate of 8-17 DNAzyme.
BBa_K3696000, BBa_K3696002--BBa_K3696003 are used in HTDC testings, the protocol is shown below:
The system is put into the enzyme marker and reacted for 60 minutes at 37℃.
From the detected fluorescence, we determine that: HTDC system's detection limit for Pb2+ is 1 nM.
BBa_K3696001, BBa_K3696002 are used in 8-17 DNAzyme testings, the protocol is shown below:
The system is put into the enzyme marker and reacted for 60 minutes at 37℃.
From the detected fluorescence, we determine that: 8-17 DNAzyme system's detection limit for Pb2+ is 10 nM.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]